RNA

Part:BBa_K2254130:Design

Designed by: Ching Yuet To   Group: iGEM17_Hong_Kong-CUHK   (2017-08-31)


N1-3 Toehold Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 680
    Illegal AgeI site found at 792
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The toehold switch was designed by using the online software designed by iGEM17 Hong Kong-CUHK team. The H5 RNA sequence (A/Egypt/MOH_NRC_8434/2014) was first downloaded from the Influenza Research Database and inputted into the program. The toehold switch with the highest ΔG RBS-Linker was chosen.


Source

This part is synthesized by the gBlock synthesis service provided by the IDT company.

References